prp4 from Schizosaccharomyces pombe, a mutant deficient in pre-mRNA splicing isolated using genes containing artificial introns.

doi:10.1007/BF00273617

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Article

prp4 from Schizosaccharomyces pombe, a mutant deficient in pre-mRNA splicing isolated using genes containing artificial introns.

Molecular & general genetics : MGG - 1 Apr 1991

Rosenberg G H, Alahari S K, Käufer N F

Abstract excerpt

We have generated a bank of temperature-sensitive (ts) Schizosaccharomyces pombe mutant strains. About 150 of these mutants were transformed with a ura4 gene containing an artificial intron. We screened these ts mutants for mutants deficient in splicing of the ura4 intron. With this approach thre...

Topics

Genes, Fungal
Genes, Synthetic
Genetic Complementation Test
Introns
Mutation
RNA Precursors
RNA Splicing
RNA, Fungal
Schizosaccharomyces
Temperature

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