Article
Isolation of an essential Schizosaccharomyces pombe gene, prp31(+), that links splicing and meiosis.
Nucleic acids research - 1 Jun 2000
Bishop D T, McDonald W H, Gould K L, Forsburg S L
Abstract excerpt
We carried out a screen for mutants that arrest prior to premeiotic S phase. One of the strains we isolated contains a temperature-sensitive allele mutation in the fission yeast prp31(+) gene. The prp31-E1 mutant is defective in vegetative cell growth and in meiotic progression. It is synthetically lethal with prp6 and displays a pre-mRNA splicing defect at the restrictive temperature. We cloned the wild-type...
Topics
- Amino Acid Sequence
- Cloning, Molecular
- Fungal Proteins
- Genes, Fungal
- Genes, Lethal
- Genetic Complementation Test
- Meiosis
- Molecular Sequence Data
- Mutation
- Phenotype
- RNA Precursors
- RNA Splicing
- Ribonucleoprotein, U4-U6 Small Nuclear
- Ribonucleoprotein, U5 Small Nuclear