Article
The enhancer of the human transferrin gene is organized in two structural and functional domains.
The Journal of biological chemistry - 25 May 1991
Boissier F, Augé-Gouillou C, Schaeffer E, Zakin M M
Abstract excerpt
We previously identified a 300-base pair long enhancer, located 3.6 kilobases upstream of the cap site of the human transferrin gene. A 5' deletion up to position 86 of the enhancer resulted in complete loss of the enhancer activity. Here we show by competition footprint analysis, gel retardation assays, and transient expression studies in hepatoma and HeLa cells that the enhancer is composed of two distinct...
Topics
- Base Sequence
- Binding Sites
- Chloramphenicol O-Acetyltransferase
- DNA Fingerprinting
- Enhancer Elements, Genetic
- HeLa Cells
- Humans
- Molecular Sequence Data
- Mutation
- Plasmids
- Transferrin