Article
Carboxypeptidase yscS: gene structure and function of the vacuolar enzyme.
European journal of biochemistry - 23 Apr 1991
Spormann D O, Heim J, Wolf D H
Abstract excerpt
The gene encoding carboxypeptidase yscS in Saccharomyces cerevisiae, CPS1, was cloned by complementation of the cps1-3 mutation. The cloned CPS1 gene, which again enabled a leucine auxotrophic cps1-3 mutant to grow on the modified dipeptide Cbz-Gly-Leu (Cbz, benzyloxycarbonyl) as sole leucine source, was sequenced and found to consist of an open reading frame of 1728 bp encoding a protein of 576 amino acids. The...
Topics
- Amino Acid Sequence
- Base Sequence
- Blotting, Northern
- Carboxypeptidases
- Chromosomes, Fungal
- DNA, Fungal
- Glycosylation
- Molecular Sequence Data
- Mutation
- RNA, Fungal
- Restriction Mapping