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Initial Colony Morphology-Based Selection for iPS Cells Derived from Adult Fibroblasts Is Substantially Improved by Temporary UTF1-Based Selection

5 Mar 2010

Abstract excerpt

BACKGROUND: Somatic cells can be reprogrammed into induced pluripotent stem (iPS) cells. Recently, selection of fully reprogrammed cells was achieved based on colony morphology reminiscent of embryonic stem (ES) cells. The maintenance of pluripotency was analysed. METHODOLOGY/PRINCIPAL FINDINGS: Clonal murine iPS cell line TiB7-4, which was derived from adult fibroblasts, was analysed for maintenance of...

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PMID: 20221450
DOI: 10.1371/journal.pone.0009580

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