Article
Ion-exchange capture of labeled pyrrolidinyl peptide nucleic acids for DNA sequence determination.
Nucleic acids symposium series (2004) - 1 Jan 2008
Korkaew Pratchayaporn, Vilaivan Tirayut
Abstract excerpt
Peptide nucleic acid (PNA) is a new class of DNA mimic, which is potentially useful for diagnostic and therapeutic applications due to its high affinity and specificity of binding to target nucleic acids. The goal of this work is to develop a simple and sensitive fluorimetric-based detection of DNA sequences using new fluorescent labeled PNA probes previously developed in our laboratory. The method relies on the...
Topics
- Base Pair Mismatch
- DNA
- Fluorescent Dyes
- Humans
- Ion Exchange
- Microscopy, Fluorescence
- Oligonucleotide Probes
- Peptide Nucleic Acids
- Polymorphism, Single Nucleotide
- Sequence Analysis, DNA