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Article

Rapid construction and characterization of synthetic antibody libraries without DNA amplification.

Biotechnology and bioengineering - 15 Jun 2010

Ge Xin, Mazor Yariv, Hunicke-Smith Scott P, Ellington Andrew D, Georgiou George

Abstract excerpt

We report on a simple method to rapidly generate very large libraries of genes encoding mutant proteins without the use of DNA amplification, and the application of this methodology in the construction of synthetic immunoglobulin variable heavy (V(H)) and light (V(kappa)) libraries. Four high quality, chemically synthesized polynucleotides (90-140 bases) were annealed and extended using T4 DNA polymerase....

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 20198660
DOI: 10.1002/bit.22712

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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