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Article

Rapid construction and characterization of synthetic antibody libraries without DNA amplification.

Biotechnology and bioengineering - 15 Jun 2010

Ge Xin, Mazor Yariv, Hunicke-Smith Scott P, Ellington Andrew D, Georgiou George

Abstract excerpt

We report on a simple method to rapidly generate very large libraries of genes encoding mutant proteins without the use of DNA amplification, and the application of this methodology in the construction of synthetic immunoglobulin variable heavy (V(H)) and light (V(kappa)) libraries. Four high qua...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 20198660
DOI: 10.1002/bit.22712

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-20198660-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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