Semisynthetic combinatorial antibody libraries: a chemical solution to the diversity problem.

doi:10.1073/pnas.89.10.4457

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Back to search

Article

Semisynthetic combinatorial antibody libraries: a chemical solution to the diversity problem.

Proceedings of the National Academy of Sciences of the United States of America - 15 May 1992

Barbas C F, Bain J D, Hoekstra D M, Lerner R A

Abstract excerpt

The properties of naiveté and large diversity are considered to be essential starting features for combinatorial antibody libraries that eschew immunization by evolution in vitro. We have prepared large libraries with such properties by using random oligonucleotide synthesis, which has the potential to create approximately 10(20) complementarity-determining regions for antibody heavy chains. When combined with...

Topics

Amino Acid Sequence
Antibody Diversity
Antibody Specificity
Base Sequence
Binding Sites, Antibody
Binding, Competitive
Enzyme-Linked Immunosorbent Assay
Escherichia coli
Gene Library
Genetic Variation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Humans

Immunoglobulin Fab Fragments

Immunoglobulin Heavy Chains

Molecular Sequence Data

Oligodeoxyribonucleotides

Plasmids

Source

Open in PubMed Open full text

Identifiers

PMID: 1584777
DOI: 10.1073/pnas.89.10.4457
PMCID: PMC49101

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1584777-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Semisynthetic combinatorial antibody libraries: a chemical solution to the diversity problem.PMID 1584777Read ↗

Design of synthetic antibody librariesSemantic proximity Read ↗

Synthetic antibodies designed on natural sequence landscapes.Semantic proximity Read ↗

Human antibody libraries: A race to engineer and explore a larger diversitySemantic proximity Read ↗

Chemical Diversification of Simple Synthetic AntibodiesSemantic proximity Read ↗

Construction of semisynthetic antibody libraries.Semantic proximity Read ↗

Creation of the large and highly functional synthetic repertoire of human VH and Vκ domain antibodies.Semantic proximity Read ↗

The Human Combinatorial Antibody Library HuCAL GOLD Combines Diversification of All Six CDRs According to the Natural Immune System with a Novel Display Method for Efficient Selection of High-Affinity AntibodiesSemantic proximity Read ↗

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody LibrariesSemantic proximity Read ↗

Drug-like antibodies with high affinity, diversity and developability directly from next-generation antibody librariesSemantic proximity Read ↗

A Novel Heavy Domain Antibody Library with Functionally Optimized Complementarity Determining RegionsSemantic proximity Read ↗

Picomolar affinity antibodies from a fully synthetic naive library selected and evolved by ribosome display.Semantic proximity Read ↗

Generation, Diversity Determination, and Application to Antibody Selection of a Human Naïve Fab LibrarySemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.