Article
Tapping diversity lost in transformations--in vitro amplification of ligation reactions.
Nucleic acids research - 1 Jan 2006
Christ Daniel, Famm Kristoffer, Winter Greg
Abstract excerpt
Molecular evolution is a powerful means of engineering proteins. It usually requires the generation of a large recombinant DNA library of variants for cloning into a phage or plasmid vector, and the transformation of a host organism for expression and screening of the variant proteins. However, library size is often limited by the low yields of circular DNA and the poor transformation efficiencies of linear DNA....
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