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Rapid Categorization of Pathogenic <i>Escherichia coli</i> by Multiplex PCR

1 Jun 2005

Abstract excerpt

A one-shot multiplex polymerase chain reaction (PCR) was developed for detecting 12 virulence genes of diarrheagenic Escherichia coli. In order to differentiate between the five categories of diarrheagenic E. coli, we selected the target genes: stx1, stx2, and eaeA for enterohemorrhagic E. coli(EHEC); eaeA, bfpA, and EAF for enteropathogenic E. coli(EPEC); invE for enteroinvasive E. coli(EIEC); elt, estp, and...

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Identifiers

PMID: 15965295
DOI: 10.1111/j.1348-0421.2005.tb03752.x

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