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Article

Real-time multiplex PCR assay and melting curve analysis for identifying diarrheagenic Escherichia coli.

Journal of clinical microbiology - 1 Mar 2013

Souza Tamara B, Lozer Diego M, Kitagawa Sônia M S, Spano Liliana C, Silva Neusa P, Scaletsky Isabel C A

Abstract excerpt

A real-time multiplex PCR assay was designed to amplify the virulence genes eae, pEAF, aatA, daaC, elt, est, ipaH, stx(1), and stx(2) for the detection of all diarrheagenic Escherichia coli pathotypes. This assay proved to be more sensitive and rapid than a conventional multiplex PCR for diarrheagenic E. coli isolates from children with diarrhea.

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Source

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Identifiers

PMID: 23303494
DOI: 10.1128/JCM.02478-12
PMCID: PMC3592053

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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