Detection of Shiga-Like Toxin ( <i> stx ₁ </i> and <i> stx ₂ </i> ), Intimin ( <i>eaeA</i> ), and Enterohemorrhagic <i>Escherichia coli</i> (EHEC) Hemolysin (EHEC <i>hlyA</i> ) Genes in Animal Feces by Multiplex PCR

Abstract excerpt

A multiplex PCR was developed for the rapid detection of genes encoding Shiga toxins 1 and 2 (stx1 and stx2), intimin (eaeA), and enterohemolysin A (hlyA) in 444 fecal samples derived from healthy and clinically affected cattle, sheep, pigs, and goats. The method involved non-solvent-based extraction of nucleic acid from an aliquot of an overnight culture of feces in EC (modified) broth. The detection limit of...

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