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Multiplex PCR for detection of trait and virulence factors in enterohemorrhagic Escherichia coli serotypes.

Molecular and cellular probes - 1 Dec 2000

Feng P, Monday S R

Abstract excerpt

A multiplex PCR assay was developed which allowed the simultaneous detection of five trait genes or virulence markers in enterohemorrhagic Escherichia coli (EHEC) serotypes. A primer pair, designed to detect a single base-pair mutation in the uidA gene, is specific only for the prototypic EHEC of O157:H7 serotype and its toxigenic, non-motile variants. In a similar way, primers to the eaeA gene of the...

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PMID: 11090262
DOI: 10.1006/mcpr.2000.0323

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