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Article

Enhancement of the aspartame precursor synthetic activity of an organic solvent-stable protease.

Protein engineering, design & selection : PEDS - 1 Mar 2010

Ogino Hiroyasu, Tsuchiyama Shotaro, Yasuda Masahiro, Doukyu Noriyuki

Abstract excerpt

The PST-01 protease is highly stable and catalyzes the synthesis of the aspartame precursor with high reaction yields in the presence of organic solvents. However, the synthesis rate using the PST-01 protease was slower than that observed when thermolysin was used. Structural comparison of both enzymes showed particular amino acid differences near the active center. These few residue differences in the PST-01...

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PMID: 20083492
DOI: 10.1093/protein/gzp086

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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