Article
Characterization of the F plasmid mating aggregation gene traN and of a new F transfer region locus trbE.
Journal of molecular biology - 20 May 1992
Maneewannakul S, Kathir P, Ippen-Ihler K
Abstract excerpt
The product of the F plasmid transfer gene, traN, is thought to be required for the formation of stable mating aggregates during F-directed conjugation. By testing chimeric plasmids that express F transfer region segments for complementation of F lac traN mutant transfer, we mapped traN to the F transfer region between trbC and traF. Both protein and DNA sequence analysis determined the traN product to be a...
Topics
- Amino Acid Sequence
- Bacterial Outer Membrane Proteins
- Bacterial Proteins
- Base Sequence
- Cloning, Molecular
- Conjugation, Genetic
- Escherichia coli Proteins
- F Factor
- Membrane Proteins
- Molecular Sequence Data
- Mutation
