Article
X-ray absorption studies of Zn(2+)-binding sites in Escherichia coli transhydrogenase and its betaH91K mutant.
Biochimica et biophysica acta - 1 Apr 2010
Veronesi Giulia, Whitehead Simon J, Francia Francesco, Giachini Lisa, Boscherini Federico, Venturoli Giovanni, Cotton Nick P J, Jackson J Baz
Abstract excerpt
Transhydrogenase couples hydride transfer between NADH and NADP(+) to proton translocation across a membrane. The binding of Zn(2+) to the enzyme was shown previously to inhibit steps associated with proton transfer. Using Zn K-edge X-ray absorption fine structure (XAFS), we report here on the local structure of Zn(2+) bound to Escherichia coli transhydrogenase. Experiments were performed on wild-type enzyme and...
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