Article
Depleting gene activities in early Drosophila embryos with the "maternal-Gal4-shRNA" system.
Genetics - 1 Jan 2013
Staller Max V, Yan Dong, Randklev Sakara, Bragdon Meghan D, Wunderlich Zeba B, Tao Rong, Perkins Lizabeth A, Depace Angela H, Perrimon Norbert
Abstract excerpt
In a developing Drosophila melanogaster embryo, mRNAs have a maternal origin, a zygotic origin, or both. During the maternal-zygotic transition, maternal products are degraded and gene expression comes under the control of the zygotic genome. To interrogate the function of mRNAs that are both maternally and zygotically expressed, it is common to examine the embryonic phenotypes derived from female germline...
Topics
- Animals
- Drosophila Proteins
- Drosophila melanogaster
- Female
- Gene Dosage
- Gene Expression Regulation, Developmental
- Gene Knockdown Techniques
- Male
- Oogenesis
- Phenotype
- RNA Interference
- RNA, Small Interfering
- Transcription Factors