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Article

STIM1 gates the store-operated calcium channel ORAI1 in vitro.

Nature structural & molecular biology - 1 Jan 2010

Zhou Yubin, Meraner Paul, Kwon Hyoung T, Machnes Danya, Oh-hora Masatsugu, Zimmer Jochen, Huang Yun, Stura Antonio, Rao Anjana, Hogan Patrick G

Abstract excerpt

Store-operated Ca(2+) entry through the plasma membrane Ca(2+) release-activated Ca(2+) (CRAC) channel in mammalian T cells and mast cells depends on the sensor protein stromal interaction molecule 1 (STIM1) and the channel subunit ORAI1. To study STIM1-ORAI1 signaling in vitro, we have expressed human ORAI1 in a sec6-4 strain of the yeast Saccharomyces cerevisiae and isolated sealed membrane vesicles carrying...

Topics

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Calcium
Calcium Channels
Chromatography, Gel
Ion Channel Gating
Neoplasm Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Secretory Vesicles

Stromal Interaction Molecule 1

Source

Open in PubMed Open full text

Identifiers

PMID: 20037597
DOI: 10.1038/nsmb.1724
PMCID: PMC2902271

Genes

ORAI1
STIM1

Variants

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 1 Sept 2026.

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STIM1 gates the store-operated calcium channel ORAI1 in vitro.PMID 20037597Read ↗

Differential roles of the C and N termini of Orai1 protein in interacting with stromal interaction molecule 1 (STIM1) for Ca2+ release-activated Ca2+ (CRAC) channel activation.Semantic proximity Read ↗

Orai1 is an essential pore subunit of the CRAC channel.Semantic proximity Read ↗

Mutations of the Ca2+-sensing stromal interaction molecule STIM1 regulate Ca2+ influx by altered oligomerization of STIM1 and by destabilization of the Ca2+ channel Orai1.Semantic proximity Read ↗

SOAR and the polybasic STIM1 domains gate and regulate Orai channels.Semantic proximity Read ↗

Gated regulation of CRAC channel ion selectivity by STIM1.Semantic proximity Read ↗

Cholesterol modulates Orai1 channel function.Semantic proximity Read ↗

Orais and STIMs: physiological mechanisms and disease.Semantic proximity Read ↗

Orai channel pore properties and gating by STIM: implications from the Orai crystal structure.Semantic proximity Read ↗

STIM1 carboxyl-terminus activates native SOC, I(crac) and TRPC1 channels.Semantic proximity Read ↗

Activation of STIM1-Orai1 involves an intramolecular switching mechanism.Semantic proximity Read ↗

Combined immunodeficiency due to a homozygous mutation in ORAI1 that deletes the C-terminus that interacts with STIM 1.Semantic proximity Read ↗

Intramolecular shielding maintains the ER Ca²⁺ sensor STIM1 in an inactive conformation.Semantic proximity Read ↗

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