Article
[Biological character of porcine circovirus type 2 with site mutation at interferon stimulation reaction element (ISRE) like region in Rep gene promoter].
Wei sheng wu xue bao = Acta microbiologica Sinica - 1 Sept 2009
Gu Jinyan, Cao Ruibing, Zhang Yu, Chen Puyan
Abstract excerpt
OBJECTIVE: To explore the biological function of the interferon stimulation reaction element (ISRE) like motif CTGAAAACGAAAGA within porcine circovirus type 2 (PCV2) Rep promoter. METHODS: Two recombinant PCV2 strains, namely PCV2 1740(G-C) and PCV2 1741(A-T), were constructed by transfecting PK15 cells with site-mutated infectious clone of PCV2 strain Denta. Replication character, genetic stability and reactive...
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