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Article

Profiling non-lysyl tRNAs in HIV-1

9 Dec 2009

Abstract excerpt

During its assembly, human HIV-1 selectively packages the tRNA(Lys) isoacceptors, including tRNA(Lys3), the primer for the reverse transcriptase. However, other low molecular weight RNA species are also seen in the virus. We profiled the tRNAs packaged into HIV-1 using microarray analysis and validated our results by two-dimensional gel electrophoresis and RT-PCR. In addition to tRNA(Lys) isoacceptors, tRNA(Asn)...

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PMID: 20007329
DOI: 10.1261/rna.1928110

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