Article
Multilayering and loss of apical polarity in MDCK cells transformed with viral K-ras.
The Journal of cell biology - 1 Mar 1991
Schoenenberger C A, Zuk A, Kendall D, Matlin K S
Abstract excerpt
The effects of viral Kirsten ras oncogene expression on the polarized phenotype of MDCK cells were investigated. Stable transformed MDCK cell lines expressing the v-K-ras oncogene were generated via infection with a helper-independent retroviral vector construct. When grown on plastic substrata, transformed cells formed continuous monolayers with epithelial-like morphology. However, on permeable filter supports...
Topics
- Animals
- Cadherins
- Cell Adhesion
- Cell Communication
- Cell Division
- Cell Line, Transformed
- Cell Membrane
- Cell Transformation, Neoplastic
- Fluorescent Antibody Technique
- Genes, ras
- Intercellular Junctions