Spatiotemporal expression of catenins, ZO-1, and occludin during early polarization of hepatic WIF-B9 cells.

doi:10.1152/ajpcell.2001.280.3.C527

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Article

Spatiotemporal expression of catenins, ZO-1, and occludin during early polarization of hepatic WIF-B9 cells.

American journal of physiology. Cell physiology - 1 Mar 2001

Decaens C, Cassio D

Abstract excerpt

WIF-B9 is a suitable model for in vitro studies of hepatocyte polarity. To better understand polarity establishment, we have localized key proteins of the adhesion system, cytoskeleton, and tight junctions soon after plating, when most cells are isolated or in doublets. In isolated attached cells, only cytoskeletal proteins (tubulin, cytokeratins) displayed a precise localization. As soon as two cells formed a...

Topics

Animals
Cadherins
Cell Line
Cell Polarity
Epithelial Cells
Humans
Hybrid Cells
Liver
Membrane Proteins
Occludin
Phenotype
Phosphoproteins
Rats
Solubility
Time Factors
Tissue Distribution
Zonula Occludens-1 Protein

Source

Open in PubMed

Identifiers

PMID: 11171572
DOI: 10.1152/ajpcell.2001.280.3.C527

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