Analysis of an anomalous mutant of MutM DNA glycosylase leads to new insights into the catalytic mechanism.

Abstract excerpt

To determine the factors involved in the specific recognition function of a bacterial 8-oxoguanine (oxoG) DNA glycosylase MutM, a series of potentials of mean force and thermodynamic integration simulations were performed with the wild type and a single-point E3Q mutant of MutM bound to oxoG and G-containing DNA, respectively. Interestingly, the mutation of the catalytically important Glu3 (E3) residue to Gln (Q)...

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