Article
Improving the photostability of bright monomeric orange and red fluorescent proteins.
Nature methods - 1 Jun 2008
Shaner Nathan C, Lin Michael Z, McKeown Michael R, Steinbach Paul A, Hazelwood Kristin L, Davidson Michael W, Tsien Roger Y
Abstract excerpt
All organic fluorophores undergo irreversible photobleaching during prolonged illumination. Although fluorescent proteins typically bleach at a substantially slower rate than many small-molecule dyes, in many cases the lack of sufficient photostability remains an important limiting factor for experiments requiring large numbers of images of single cells. Screening methods focusing solely on brightness or...
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