Article
Kinetic and structural characterization of dihydrofolate reductase from Streptococcus pneumoniae.
Biochemistry - 12 Jan 2010
Lee Jeeyeon, Yennawar Neela H, Gam Jongsik, Benkovic Stephen J
Abstract excerpt
Drug resistance associated with dihydrofolate reductase (DHFR) has emerged as a critical issue in the treatment of bacterial infections. In our efforts to understand the mechanism of a drug-resistant dihydrofolate reductase (DHFR) from a pathogenic bacterial source, we report the first kinetic characterization of Streptococcus pneumoniae DHFR (spDHFR) along with its X-ray structure. This study revealed that the...
Topics
- Amino Acid Sequence
- Binding Sites
- Catalytic Domain
- Crystallography, X-Ray
- G-Quadruplexes
- Histidine
- Hydrogen-Ion Concentration
- Kinetics
- Models, Molecular
- Molecular Sequence Data
- Mutation