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Article

A hydrogen-bonding network is important for oxidation and isomerization in the reaction catalyzed by cholesterol oxidase.

Acta crystallographica. Section D, Biological crystallography - 1 Nov 2009

Lyubimov Artem Y, Chen Lin, Sampson Nicole S, Vrielink Alice

Abstract excerpt

Cholesterol oxidase is a flavoenzyme that catalyzes the oxidation and isomerization of 3beta-hydroxysteroids. Structural and mutagenesis studies have shown that Asn485 plays a key role in substrate oxidation. The side chain makes an NH...pi interaction with the reduced form of the flavin cofactor. A N485D mutant was constructed to further test the role of the amide group in catalysis. The mutation resulted in a...

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Identifiers

PMID: 19923719
DOI: 10.1107/S0907444909037421
PMCID: PMC3089011

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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