Article
Studies on a Tyr residue critical for the binding of coenzyme and substrate in mouse 3(17)alpha-hydroxysteroid dehydrogenase (AKR1C21): structure of the Y224D mutant enzyme.
Acta crystallographica. Section D, Biological crystallography - 1 Feb 2010
Dhagat Urmi, Endo Satoshi, Mamiya Hiroaki, Hara Akira, El-Kabbani Ossama
Abstract excerpt
Mouse 3(17)alpha-hydroxysteroid dehydrogenase (AKR1C21) is the only aldo-keto reductase that catalyzes the stereospecific reduction of 3- and 17-ketosteroids to the corresponding 3(17)alpha-hydroxysteroids. The Y224D mutation of AKR1C21 reduced the K(m) value for NADP(H) by up to 80-fold and comp...
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