Article
Hairpin formation in the self-complementary dodecamer d-GGTACGCGTACC and derivatives containing GA and IA mispairs.
Biochemistry - 22 Jan 1991
Howard F B, Chen C Q, Ross P D, Miles H T
Abstract excerpt
The dodecamer d-GGTACGCGTACC and four derivatives with GA and IA mispairs in the 6,7 and 5,8 positions have been examined in dilute solution and 0.01-0.1 M sodium chloride. Concentration dependence of Tm, gel electrophoresis, and equilibrium centrifugation indicate that these self-complementary oligomers can form hairpins under the present conditions. Thermal transitions measured in the ultraviolet primarily...
Topics
- Base Composition
- Base Sequence
- DNA
- Deoxyribonucleotides
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Temperature
- Thermodynamics