Article
Dissection of functional domains in Escherichia coli DNA photolyase by linker-insertion mutagenesis.
Molecular & general genetics : MGG - 1 Mar 1992
Yamamoto K
Abstract excerpt
The phr gene, which encodes protein of 472 amino acid residues, is required for light-dependent photoreactivation and enhances light-independent excision repair of ultraviolet light (UV)-induced DNA damage. In this study, dodecamer HindIII linker insertions were introduced into the cloned phr gene and the functional effects of the resulting mutations on photoreactivation and light-independent dark repair in vivo...
Topics
- Amino Acid Sequence
- Base Sequence
- DNA Mutational Analysis
- DNA Repair
- Deoxyribodipyrimidine Photo-Lyase
- Escherichia coli
- Immunoblotting
- Molecular Sequence Data
- Mutagenesis, Insertional
- Mutation
- Pyrimidine Dimers