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Article

Detection of gyrA and gyrB mutations in Clostridium difficile isolates by real-time PCR.

Molecular and cellular probes - 1 Apr 2010

Spigaglia Patrizia, Carattoli Alessandra, Barbanti Fabrizio, Mastrantonio Paola

Abstract excerpt

Fluoroquinolone (FQ)-resistance in Clostridium difficile has been associated with mutations in the quinolone-resistance determining region (QRDR) of gyr genes. In particular, the majority of resistant clinical isolates show mutations in codon 82 of gyrA or in codon 426 of gyrB. A real-time PCR method was developed to identify these mutations in FQ-resistant C. difficile strains. Twenty-one clinical isolates,...

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Identifiers

PMID: 19837154
DOI: 10.1016/j.mcp.2009.10.002

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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