Article
Development of a real-time PCR assay for detection of gyrA mutations associated with reduced susceptibility to ciprofloxacin in Salmonella enterica serovar typhi and paratyphi A.
Microbiology and immunology - 1 Jan 2006
Morita Masatomo, Ito Kenitiro, Hirose Kenji, Takahashi Hideyuki, Shimuta Ken, Terajima Jun, Ohnishi Makoto, Harada Makoto, Matsuzaki Mitsuhiro, Watanabe Haruo, Izumiya Hidemasa
Abstract excerpt
A real-time PCR assay with the cycling probe method was used to detect mutations at codons 83 and 87 in the DNA gyrase A subunit encoded by gyrA in Salmonella enterica serovar Typhi and Paratyphi A clinical isolates. The susceptibility estimated from the results of the gyrA mutation assay was con...
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