Article
Cloning and nucleotide sequence of Mycobacterium tuberculosis gyrA and gyrB genes and detection of quinolone resistance mutations.
Antimicrobial agents and chemotherapy - 1 Apr 1994
Takiff H E, Salazar L, Guerrero C, Philipp W, Huang W M, Kreiswirth B, Cole S T, Jacobs W R, Telenti A
Abstract excerpt
The emergence of multidrug-resistant strains of Mycobacterium tuberculosis has resulted in increased interest in the fluoroquinolones (FQs) as antituberculosis agents. To investigate the frequency and mechanisms of FQ resistance in M. tuberculosis, we cloned and sequenced the wild-type gyrA and gyrB genes, which encode the A and B subunits of the DNA gyrase, respectively; DNA gyrase is the main target of the FQs....
Topics
- Anti-Infective Agents
- Base Sequence
- Ciprofloxacin
- Cloning, Molecular
- DNA Topoisomerases, Type II
- Drug Resistance, Microbial
- Genes, Bacterial
- Humans
- Molecular Sequence Data
- Mutation