Article
Real-time PCR assay for detection of fluoroquinolone resistance associated with grlA mutations in Staphylococcus aureus.
Journal of clinical microbiology - 1 Jul 2003
Lapierre Pascal, Huletsky Ann, Fortin Véronique, Picard François J, Roy Paul H, Ouellette Marc, Bergeron Michel G
Abstract excerpt
Resistance to fluoroquinolones among clinical isolates of Staphylococcus aureus has become a clinical problem. Therefore, a rapid method to identify S. aureus and its susceptibility to fluoroquinolones could provide clinicians with a useful tool for the appropriate use of these antimicrobial agents in the health care settings. In this study, we developed a rapid real-time PCR assay for the detection of S. aureus...
Topics
- Anti-Infective Agents
- DNA Topoisomerase IV
- Drug Resistance, Bacterial
- Fluoroquinolones
- Humans
- Microbial Sensitivity Tests
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- Sensitivity and Specificity
- Sequence Analysis, DNA
- Staphylococcus aureus