Article
Detection of parC mutations in Streptococcus pneumoniae by Real-time PCR and Taqman-MGB probes.
Journal of microbiological methods - 1 Apr 2007
Alonso Rodrigo, Mateo Estibaliz, Cisterna Ramón
Abstract excerpt
A Real-time PCR assay was developed by using Taqman-MGB probes to screen mutations at codons Ser79 and Asp83 of Streptococcus pneumoniae parC. One hundred and thirty levofloxacin-susceptible and forty-two levofloxacin-resistant clinical strains were assayed. Mutations at codon 79 were found among all the levofloxacin-resistant strains. Mutations at codon 79 or 83 were found in ten levofloxacin-susceptible...
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