Article
The interaction of spinach nitrite reductase with ferredoxin: a site-directed mutation study.
Molecular plant - 1 May 2009
Hirasawa Masakazu, Tripathy Jatindra N, Somasundaram Ramasamy, Johnson Michael K, Bhalla Megha, Allen James P, Knaff David B
Abstract excerpt
A series of site-directed mutants of the ferredoxin-dependent spinach nitrite reductase has been characterized and several amino acids have been identified that appear to be involved in the interaction of the enzyme with ferredoxin. In a complementary study, binding constants to nitrite reductase and steady-state kinetic parameters of site-directed mutants of ferredoxin were determined in an attempt to identify...
Topics
- Binding Sites
- Conserved Sequence
- Ferredoxin-Nitrite Reductase
- Ferredoxins
- Mutagenesis, Site-Directed
- Mutation
- Nitrite Reductases
- Sequence Alignment
- Sequence Homology, Amino Acid
- Spinacia oleracea