Article
Nitrate reductase of Neurospora crassa: the functional role of individual amino acids in the heme domain as examined by site-directed mutagenesis.
Molecular & general genetics : MGG - 1 Aug 1993
Okamoto P M, Marzluf G A
Abstract excerpt
The enzyme nitrate reductase, which catalyzes the reduction of nitrate to nitrite, is a multi-redox center homodimeric protein. Each polypeptide subunit is approximately 100 kDa in size and contains three separate domains, one each for a flavin, a heme-iron, and a molybdopterin cofactor. The heme...
Topics
- Amino Acid Sequence
- Amino Acids
- Binding Sites
- Blotting, Southern
- Enzyme Stability
- Heme
- Histidine
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Neurospora crassa
- Nitrate Reductase
- Nitrate Reductases
- Phenotype
- Protein Structure, Tertiary
- Transformation, Genetic