Article
Flavodoxin-mediated electron transfer from photosystem I to ferredoxin-NADP+ reductase in Anabaena: role of flavodoxin hydrophobic residues in protein-protein interactions.
Biochemistry - 29 Jan 2008
Goñi Guillermina, Serrano Ana, Frago Susana, Hervás Manuel, Peregrina José Ramón, De la Rosa Miguel A, Gómez-Moreno Carlos, Navarro José A, Medina Milagros
Abstract excerpt
Three surface hydrophobic residues located at the Anabaena flavodoxin (Fld) putative complex interface with its redox partners were replaced by site-directed mutagenesis. The effects of these replacements on Fld interaction with both its physiological electron donor, photosystem I (PSI), and its electron acceptor, ferredoxin-NADP+ reductase (FNR), were analyzed. Trp57, Ile59, and Ile92 contributed to the optimal...
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