Article
Biochemical and Biophysical Methods to Examine the Effects of Site-Directed Mutagenesis on Enzymatic Activities and Interprotein Interactions.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017
Kinoshita Misaki, Kim Ju Yaen, Lin Yuxi, Markova Natalia, Hase Toshiharu, Lee Young-Ho
Abstract excerpt
Mutations in proteins often affect interactions with partner molecules, sequentially changing their activities and functions. In order to examine mutagenic effects, we herein describe practical and detailed protocols for enzymatic activity assays using ferredoxin (Fd)-NADP+ reductase (FNR) and sulfite reductase (SiR), which are electron-transferring enzymes for the Calvin cycle and sulfur assimilation in various...
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