Article
Identification and isolation of Mu-flanking fragments from maize.
Journal of genetics and genomics = Yi chuan xue bao - 1 Apr 2008
Wang Yijun, Yin Guangming, Yang Qin, Tang Jihua, Lu Xiaomin, Korban Schuyler S, Xu Mingliang
Abstract excerpt
Transposable elements have been utilized as mutagens to create mutant libraries for functional genomics. Isolation of genomic segments flanking the insertion Mutator (Mu) is a key step in insertion mutagenesis studies. Herein, we adopted a modified AFLP method to identify and isolate Mu-flanking fragments from maize. The method consists of the following steps: 1) double-digestion of genomic DNA with Bgl II/Msp I...
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