Article
Characterization of human cone phosphodiesterase-6 ectopically expressed in Xenopus laevis rods.
The Journal of biological chemistry - 20 Nov 2009
Muradov Hakim, Boyd Kimberly K, Haeri Mohammad, Kerov Vasily, Knox Barry E, Artemyev Nikolai O
Abstract excerpt
PDE6 (phosphodiesterase-6) is the effector molecule in the vertebrate phototransduction cascade. Progress in understanding the structure and function of PDE6 has been hindered by lack of an expression system of the enzyme. Here we report ectopic expression and analysis of compartmentalization and membrane dynamics of the enhanced green fluorescent protein (EGFP) fusion protein of human cone PDE6C in rods of...
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