Article
Short oligonucleotide tandem ligation assay for genotyping of single-nucleotide polymorphisms in Y chromosome.
Molecular biotechnology - 1 May 2010
Skobeltsyna Larisa M, Pyshnyi Dmitrii V, Ivanova Eugenia M, Stepanov Vadim A, Puzyrev Valery P, Dymshits Grigory M, Kharkov Vladimir N, Zarytova Valentina F
Abstract excerpt
We propose a novel universal methodology, Short Oligonucleotide Tandem Ligation Assay (SOTLA), for SNP genotyping. SOTLA is based on using a tandem of short oligonucleotide (TSO) probes consisting of three fragments: the core oligonucleotide and two flanking oligomers, one of which is immobilized onto a solid support and another one contains the biotin label. TSO is self-associated on a complementary DNA...
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