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Predicting the success of primer extension genotyping assays using statistical modeling.

Nucleic acids research - 1 Dec 2002

Yuryev Anton, Huang JianPing, Pohl Mark, Patch Robert, Watson Felicia, Bell Peter, Donaldson Miriam, Phillips Michael S, Boyce-Jacino Michael T

Abstract excerpt

Using an empirical panel of more than 20 000 single base primer extension (SNP-IT) assays we have developed a set of statistical scores for evaluating and rank ordering various parameters of the SNP-IT reaction to facilitate high-throughput assay primer design with improved likelihood of success. Each score predicts either signal magnitude from primer extension or signal noise caused by mispriming of primers and...

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PMID: 12466563
DOI: 10.1093/nar/gnf131
PMCID: PMC137986

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