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Article

Kinetic characterisation of primer mismatches in allele-specific PCR: a quantitative assessment.

Biochemical and biophysical research communications - 20 Dec 2002

Waterfall Christy M, Eisenthal Robert, Cobb Benjamin D

Abstract excerpt

A novel method of estimating the kinetic parameters of Taq DNA polymerase during rapid cycle PCR is presented. A model was constructed using a simplified sigmoid function to represent substrate accumulation during PCR in combination with the general equation describing high substrate inhibition f...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 12470637
DOI: 10.1016/s0006-291x(02)02750-x

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-12470637-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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