Article
Synthesis, purification, and characterization of an Arg152----Glu site-directed mutant of recombinant human blood clotting factor VII.
Biochemistry - 3 Apr 1990
Wildgoose P, Berkner K L, Kisiel W
Abstract excerpt
Coagulation factor VII circulates in blood as a single-chain zymogen of a serine protease and is converted to its activated two-chain form, factor VIIa, by cleavage of an internal peptide bond located at Arg152-Ile153. Previous studies using serine protease active-site inhibitors suggest that zym...
Topics
- Amino Acid Chloromethyl Ketones
- Arginine
- Base Sequence
- Cloning, Molecular
- Dansyl Compounds
- Electrophoresis, Polyacrylamide Gel
- Factor VII
- Glutamates
- Glutamic Acid
- Humans
- Molecular Sequence Data
- Mutation
- Peptide Hydrolases
- Recombinant Proteins