Article
Site-directed mutagenesis of arginine 103 and lysine 185 in the proposed glycosaminoglycan-binding site of heparin cofactor II.
The Journal of biological chemistry - 5 Jan 1990
Blinder M A, Tollefsen D M
Abstract excerpt
Inhibition of thrombin by heparin cofactor (HCII) is accelerated approximately 1000-fold by heparin or dermatan sulfate. We found recently that the mutation Arg189----His decreases the affinity of HCII for dermatan sulfate but not for heparin (Blinder, M. A., Andersson, T. R., Abildgaard, U., and...
Topics
- Amino Acid Sequence
- Arginine
- Binding Sites
- Cloning, Molecular
- Codon
- DNA
- Dermatan Sulfate
- Escherichia coli
- Glycosaminoglycans
- Heparin
- Heparin Cofactor II
- Humans
- Lysine
- Molecular Sequence Data
- Mutation
- Recombinant Proteins
- Structure-Activity Relationship
- Thrombin