Article
Expression of the mcrA gene of Escherichia coli is regulated posttranscriptionally, possibly by sequestration of the Shine-Dalgarno region.
Gene - 19 May 1995
Shivapriya R, Prasad R, Narayanan I L, Krishnaswamy S, Dharmalingam K
Abstract excerpt
The polypeptides encoded by the mcrA gene were analysed using a T7 expression system. Cloned fragments of 1.6 and 1.0 kb displayed an McrA+/RglA+ phenotype and directed synthesis of a 31-kDa polypeptide. A derivative of these clones altered at an internal HindIII site displayed an McrA+/RglA- phe...
Topics
- Bacterial Proteins
- Base Sequence
- DNA, Bacterial
- Escherichia coli
- Frameshift Mutation
- Gene Expression Regulation, Bacterial
- Genes, Bacterial
- Models, Structural
- Molecular Sequence Data
- Nucleic Acid Conformation
- Oxidoreductases
- Phenotype
- Plasmids
- RNA, Bacterial
- Restriction Mapping
- Transcription, Genetic