Article
The Saccharomyces cerevisiae vacuolar acid trehalase is targeted at the cell surface for its physiological function.
The FEBS journal - 1 Oct 2009
He Susu, Bystricky Kerstin, Leon Sebastien, François Jean M, Parrou Jean L
Abstract excerpt
Previous studies in the yeast Saccharomyces cerevisiae have proposed a vacuolar localization for Ath1, which is difficult to reconcile with its ability to hydrolyze exogenous trehalose. We used fluorescent microscopy to show that the red fluorescent protein mCherry fused to the C-terminus of Ath1, although mostly localized in the vacuole, was also targeted to the cell surface. Also, hybrid Ath1 truncates fused at...
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