Article
Identification of essential histidine residues in the active site of Escherichia coli xylose (glucose) isomerase.
Proceedings of the National Academy of Sciences of the United States of America - 1 Jan 1990
Batt C A, Jamieson A C, Vandeyar M A
Abstract excerpt
Two conserved histidine residues (His-101 and His-271) appear to be essential components in the active site of the enzyme xylose (glucose) isomerase (EC 5.3.1.5). These amino acid residues were targeted for mutagenesis on the basis of sequence homology among xylose isomerases isolated from Escher...
Topics
- Aldose-Ketose Isomerases
- Amino Acid Sequence
- Base Sequence
- Binding Sites
- Carbohydrate Epimerases
- Circular Dichroism
- Codon
- Enzyme Stability
- Escherichia coli
- Histidine
- Kinetics
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- Plasmids
- Protein Conformation
- Sequence Homology, Nucleic Acid