Cloning and characterization of the asd gene of Salmonella typhimurium: use in stable maintenance of recombinant plasmids in Salmonella vaccine strains.

doi:10.1016/0378-1119(90)90464-3

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Article

Cloning and characterization of the asd gene of Salmonella typhimurium: use in stable maintenance of recombinant plasmids in Salmonella vaccine strains.

Gene - 28 Sept 1990

Galán J E, Nakayama K, Curtiss R

Abstract excerpt

The asd mutants of Salmonella typhimurium have an obligate requirement for diaminopimelic acid (DAP) and will undergo lysis in environments deprived of DAP. This has allowed the development of a balanced-lethal system for the expression of heterologous antigens in vaccine strains using vectors co...

Topics

Amino Acid Sequence
Bacterial Vaccines
Base Sequence
Cloning, Molecular
Escherichia coli
Genes, Bacterial
Genes, Lethal
Genotype
Molecular Sequence Data
Plasmids
Restriction Mapping
Salmonella typhimurium

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