Article
Defined single-gene and multi-gene deletion mutant collections in Salmonella enterica sv Typhimurium.
PloS one - 1 Jan 2014
Porwollik Steffen, Santiviago Carlos A, Cheng Pui, Long Fred, Desai Prerak, Fredlund Jennifer, Srikumar Shabarinath, Silva Cecilia A, Chu Weiping, Chen Xin, Canals Rocío, Reynolds M Megan, Bogomolnaya Lydia, Shields Christine, Cui Ping, Guo Jinbai, Zheng Yi, Endicott-Yazdani Tiana, Yang Hee-Jeong, Maple Aimee, Ragoza Yury, Blondel Carlos J, Valenzuela Camila, Andrews-Polymenis Helene, McClelland Michael
Abstract excerpt
We constructed two collections of targeted single gene deletion (SGD) mutants and two collections of targeted multi-gene deletion (MGD) mutants in Salmonella enterica sv Typhimurium 14028s. The SGD mutant collections contain (1), 3517 mutants in which a single gene is replaced by a cassette containing a kanamycin resistance (KanR) gene oriented in the sense direction (SGD-K), and (2), 3376 mutants with a...
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